Genome Editing with Engineered Nucleases in Plants
نویسندگان
چکیده
منابع مشابه
Genome editing with engineered nucleases in plants.
Numerous examples of successful 'genome editing' now exist. Genome editing uses engineered nucleases as powerful tools to target specific DNA sequences to edit genes precisely in the genomes of both model and crop plants, as well as a variety of other organisms. The DNA-binding domains of zinc finger (ZF) proteins were the first to be used as genome editing tools, in the form of designed ZF nuc...
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Here, we report a form of oligonucleotide-directed mutagenesis for precision genome editing in plants that uses single-stranded oligonucleotides (ssODNs) to precisely and efficiently generate genome edits at DNA strand lesions made by DNA double strand break reagents. Employing a transgene model in Arabidopsis (Arabidopsis thaliana), we obtained a high frequency of precise targeted genome edits...
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Here, we report a form of oligonucleotide-directed mutagenesis for precision genome editing in plants that uses single-stranded oligonucleotides (ssODNs) to precisely and efficiently generate genome edits at DNA strand lesions made by DNA double strand break reagents. Employing a transgene model in Arabidopsis (Arabidopsis thaliana), we obtained a high frequency of precise targeted genome edits...
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After induced mutagenesis and transgenesis, genome editing is the next step in the development of breeding techniques. Genome editing using site-directed nucleases - including meganucleases, zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the CRISPR/Cas9 system - is based on the mechanism of double strand breaks. The nuclease is directed to cleave the...
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Genome editing is an exciting technology that allows for specific manipulation of complex genomes. While the original tools for genome manipulation had low efficiencies, genome editing tools discovered in the past fifteen years have been widely studied and great efforts have been required to improve their efficiency. This article summarizes how Zinc Finger Nuclease (ZFN), Transcription Activato...
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ژورنال
عنوان ژورنال: Plant and Cell Physiology
سال: 2014
ISSN: 0032-0781,1471-9053
DOI: 10.1093/pcp/pcu170